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. 2018 Mar;88:50–60. doi: 10.1016/j.jaut.2017.10.001

Fig. 6.

Fig. 6

Capacity for phenotype change upon stimulation is reduced in SF T cells. IL-17 and IFNγ expression by CD4+ T cells from the peripheral blood and synovial fluid of RA patients was measured by flow cytometry ex vivo and after anti-CD3 stimulation for seven days. (A) Representative bidirectional FACS plots of IL-17 versus IFNγ in T cells from blood and synovial fluid (SF) from a single RA donor ex vivo and after 7 days of culture. (B) Summary of T cell frequencies in blood and synovial fluid (SF) for multiple donors ex vivo and after 7 days of culture. Significant differences were tested using Wilcoxon matched pairs tests. * = P < 0.05, ** = P < 0.01, *** = P < 0.001. (C) XY plots for frequencies of IL-17 + and IFNγ+ CD4+ T cells ex vivo and after seven days. Diagonal lines indicate equal frequencies at each time point. (D) Pie charts summarizing the frequencies of Th17 (pale grey), Th17.1 (dark grey), Th1 (black) and double negative (DN) (white) CD4+ T cells from blood and SF ex vivo and after 7 days of culture (n = 12 donors).