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. 2018 Mar 15;13(3):e0194420. doi: 10.1371/journal.pone.0194420

Table 1. Buffers used for cell lysis and immunoprecipitation for ChIP sequencing of tephritid fruit flies.

Buffer Recipe
Lysis buffer 50mM Tris-HCl pH 8.0, 1mM CaCl2, 0.2% Triton X-100, 5mM Sodium Butyrate, Protease inhibitor cocktail
RIPA buffer 10mM Tris-HCl pH 8.0, 1mM EDTA pH 8.0, 0.1% SDS, 0.1% Sodium Deoxycholate, 1% Triton X-100, Protease inhibitor cocktail
IP Dilution buffer 1% Triton X-100, 20mM Tris-HCl pH 8.0, 2mM EDTA pH 8.0, 150mM NaCl (add last), Protease inhibitor cocktail
Wash buffer 0.1% SDS, 1% Triton X-100, 20mM Tris-HCl pH 8.0, 2mM EDTA pH 8.0, 150mM NaCl (add last)
Final wash buffer 0.1% SDS, 1% Triton X-100, 20mM Tris-HCl pH 8.0, 2mM EDTA pH 8.0, 500mM NaCl (add last)
Elution buffer 1% SDS, 100mM NaHCO3