Fig 4. Depletion of ITPR3 inhibits extrusion formation.

Hela cells were treated with siRNA corresponding to ITPR3 or a scrambled negative control siRNA (Scr) for 72 hours then infected with C. trachomatis wild-type L2. (A) At 24 hr post-infection, cells were fixed and stained with anti-ITPR3 (red) (Arrowheads) and anti-MOMP (green) to verify the depletion of ITPR3. Bar = 10 μm. (B) Immunoblots confirmed knock out of ITPR3. (C) Effect on extrusion formation by siRNA depletion of the ITPR3 by siRNA. (D) Effect on total infectious progeny production by siRNA depletion of the ITPR3. Experiments were performed in triplicate (n = 3; error bars represent SEM). The difference was significant (P<0.0001; ****) by an unpaired Students T-test.