Fig 5. Cytokine expressions in CsTPs-immunized mice.
(A) Levels of IL-13, IL-4, IL-10, and IFN-γ in splenocytes of CsTPs-immunized mice by ELISA. Mice were sacrificed 2, 4, 7 or 10 weeks after CsTPs immunization and the splenocytes were isolated. 5 × 106/ml splenocytes per well were stimulated with 1× Cell Stimulation Cocktail for 48 hours and the supernatants were analyzed. (B) mRNA levels of IL-13, IL-4, IL-10, IL-12 and IFN-γ in hepatic tissue of CsTPs-immunized mice. The livers were isolated at different time points (2, 4, 7 or 10 weeks after administration). The total RNA was extracted. Specific primers were used to detect transcripts of those cytokines by RT-PCR. All data are presented as mean ± SD of triplicate wells from 3 independent experiments with 4 mice per time point per group and analyzed by calculating 2-ΔΔCt, normalized to a housekeeping gene (β-actin). Analysis by ANOVA indicated significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001) among the different groups.