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. 2018 Mar 15;14(3):e1006917. doi: 10.1371/journal.ppat.1006917

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© 2018 Poupel et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — A. The ΔspdC mutant displays increased resistance to lysostaphin-induced lysis. Cells were grown in TSB until mid-exponential phase, harvested and incubated in PBS with lysostaphin (200 ng/ml) with aeration at 37°C. Bacterial lysis was measured by monitoring OD_600nm over time. Results are shown as the mean and standard deviation of three independent assays. HG001 parental strain (); ΔspdC mutant (■); ΔspdC/pMK4Pprot-spdC complemented strain (▲). B. The absence of SpdC leads to sensitivity to oxacillin and tunicamycin. Dilution series of the HG001, ΔspdC and ΔspdC/ pMK4Pprot-spdC strains on TSA plates with or without antibiotics. Oxacillin: 0.1 μg/ml; fosfomycin: 4 μg/ml; tunicamycin: 1 μg/ml.

Inline graphic — A. The ΔspdC mutant displays increased resistance to lysostaphin-induced lysis. Cells were grown in TSB until mid-exponential phase, harvested and incubated in PBS with lysostaphin (200 ng/ml) with aeration at 37°C. Bacterial lysis was measured by monitoring OD_600nm over time. Results are shown as the mean and standard deviation of three independent assays. HG001 parental strain (); ΔspdC mutant (■); ΔspdC/pMK4Pprot-spdC complemented strain (▲). B. The absence of SpdC leads to sensitivity to oxacillin and tunicamycin. Dilution series of the HG001, ΔspdC and ΔspdC/ pMK4Pprot-spdC strains on TSA plates with or without antibiotics. Oxacillin: 0.1 μg/ml; fosfomycin: 4 μg/ml; tunicamycin: 1 μg/ml.