Skip to main content
. 2018 Mar 15;14(3):e1006917. doi: 10.1371/journal.ppat.1006917

Fig 8. Domains involved in interaction between SpdC and the WalK and SaeS histidine kinases.

Fig 8

A. Domains fused to either pUT18c or pKT25 for the BACTH assay. Hatched bars indicate transmembrane regions and functional domains are annotated according to the UniProt database (http://www.uniprot.org/uniprot/Q2G2U4 for WalK, http://www.uniprot.org/uniprot/Q2G2U1 for SaeS and http://www.uniprot.org/uniprot/Q2FVT1 for SpdC). B. β-galactosidase activities of DHT1 E. coli strains co-transformed with pUT18c containing either the full-length spdC coding sequence or a fragment encoding only the first 252 amino-acids of SpdC, and pKT25 derivatives carrying DNA fragments encoding the indicated proteins. Positive interaction results are shaded. ND: Not determined.