Table 2. Distribution of Lagos bat viral RNA and antigen in tissues of experimentally infected bats.
| Inoculum Group | Bat no. | Days to death | Tissue (lyssaviral RNA by hn-RT-PCR/lyssaviral antigen by immunohistochemistry) | Total no tissues positive hn-RT-PCR/ IHC | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Brain | Salivary gland | Tongue | Lymph nodea | Heart | Lung | Duodenum | Jejunum | Colon | Liver | Kidney | Spleen | ||||
| Senegal | 13 | 4 | + b /+ | +/- c | +/- | -/- | -/- | -/- | -/- | NAd | -/- | -/- | -/- | -/- | 3/1 |
| 5 | 6 | +/+ | +/- | -/- | -/- | +/- | +/- | -/- | -/- | +/- | -/- | +/- | -/- | 6/1 | |
| 6 | 5 | +/+ | +/- | -/-e | -/- | +/+f | +/- | +/- | -/- | -/- | -/- | -/- | -/- | 5/3 | |
| Nigeria | 7 | 8 | +/+ | -/- | -/- | -/- | -/-g | -/- | -/- | -/- | -/- | -/- | -/- | -/- | 1/1 |
| 8 | 8 | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | 1/1 | |
| 9 | 8 | +/+ | -/- | -/- | -/- | -/-g | -/- | -/- | -/- | +/- | +/- | -/- | -/- | 3/1 | |
| Ghana | 10 | 5 | +/+ | -/- | +/-h | +/- | +/+f | +/- | +/+i | +/+i | -/- | +/- | +/- | +/- | 10/5 |
| 11 | 5 | +/+ | +/- | -/- | -/- | +/+f | +/- | +/+i | +/- | +/- | -/- | -/- | +/- | 8/3 | |
| 12 | 6 | +/+ | -/+j | +/+k | -/- | +/+f | +/- | +/+i | +/- | +/- | +/- | +/- | +/- | 10/5 | |
| Total positive | 9; 9l | 4; 1 | 3; 3 | 1; 0 | 5; 4 | 5; 0 | 4; 3 | 3; 1 | 4; 0 | 3; 0 | 3; 0 | 3; 0 | |||
a Submandibular lymph node.
b +, Positive.
c -, Negative.
d NA, Sample not available.
e Taste buds positive, neurons in ganglia not.
f Neurons in ganglia of the heart contained lyssavirus antigen.
g Ganglia were not visible in heart section.
h Surface epithelium positive, neurons in ganglion not.
i Neurons in myenteric plexi positive.
j Neurons in ganglion in the interstitium of the salivary gland positive.
k Taste buds and neurons in ganglia positive.
l x; y, x indicates total number of RNA positives; y indicates total number of antigen positives.