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. 2018 Mar 5;12(3):e0006307. doi: 10.1371/journal.pntd.0006307

Fig 1. The effect of AaNotch on mosquito egg production, egg tanning and hatching.

Fig 1

(A) The number of eggs per tube after egg induction relative to dsLacZ treatment by ANOVA (** = p < 0.001). The number in the parenthesis denotes the number of mosquitoes examined. (A, C, D) Silencing efficiency based on the levels of AaNotch transcript in female mosquitoes injected with dsLacZ and dsNotch, normalized against ribosomal protein s7 transcript (bar graph on right). (B, C) Oviposited eggs from control, dsLacZ-, and dsNotch-injected female mosquitoes. Eggs with hardened and darkened surface were counted as melanized eggs, whereas eggs that remained soft and white were counted as non-melanized eggs. (B) Scale bar = 0.5 mm. (C) The percentage of melanized eggs calculated from control, dsLacZ, or dsNotch-treated mosquitoes. At least three cohorts of mosquitoes were analyzed. (D) Eggs from control, dsLacZ, or dsNotch-treated mosquitoes subjected to deoxygenation-induced hatching. The numbers of the first instar larvae were counted. Numbers in the parentheses denotes total number of mosquitoes examined.