Fig 3. A20 deletion is associated with enhanced IFNγ-mediated inhibition of tight junction and mucin gene expression.
(A) Western blot for A20 protein levels in A20FL/FL villin-ERCre+ or Cre- organoids after treatment with 4-hydroxytamoxifen (4OHT) for 24 hours. Gene expression by qPCR of (B) the proinflammatory chemokines Cxcl1 and Cxcl2, (C) the mucins Muc2, Muc4, Muc13, and Muc1, (D) the tight junction proteins Tjp1, Ocln, and Cldn4, and (E) the antimicrobial peptide Defb1 after stimulation with IFNγ for 12 hours. Statistical significance within Cre+ and Cre- conditions was first tested by ANOVA and specific pairwise comparisons were made using t-tests corrected for multiple comparisons by the Scheffe method (see also S1 Table). Stars above each column indicate statistical significance relative to the media condition. Bars with stars indicate statistically significant differences between the two indicated conditions. * P<0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.