Figure 5.
Therapeutic effect of neurosphere-pulsed DC against intracranial 9L tumor in adult rats. Rats were injected intracranially with 25000 9L glioma cells on day 0 and were vaccinated s.c. on days 7, 14, and 21 with different tumor antigen pulsed dendritic cell vaccines: control (DC only), monolayer, daughter cells, and neurospheres (n=10 for each group). Kaplan-Meier survival curve showed that rats treated with 9L neurosphere lysate-pulsed DC have longer survival than the other groups (** p=0.0015). The surviving animals with CSC vaccination were sacrificed after 70 days and slowly growing brain tumors were detected.