Table 1.
IFN-γ production after immunization with 9L-CSC-pulsed DC. Splenocytes from tumor bearing rats were re-stimulated with IL-2 for 11 days followed by re-exposure to a final 9L tumor cell target (9L monolayer cells, 9L neurospheres, or 9L daughter cells) or a blank. Only splenocytes from neurosphere-vaccinated rats released high level of IFNγ when re-exposed to neurospheres.
| Samples | Mean threshold cycles (Ct) | Mean threshold difference (ΔCt) | Fold change in normalized IFN-γ (2ΔCt) | ||
|---|---|---|---|---|---|
| CD8 | IFN-γ | ||||
| control | No target | 23.1 | 26.3 | −3.2 | 1.32 |
| Target | 23.7 | 26.5 | −2.8 | ||
| 9L-monolayer | No target | 23.4 | 24.4 | −1.0 | 1.15 |
| Target | 24.3 | 25.1 | −0.8 | ||
| 9L-daughter cells | No target | 24.8 | 28.8 | −4.0 | 1.41 |
| Target | 22.9 | 26.4 | −3.5 | ||
| 9L-neurospheres | No target | 23.2 | 28.2 | −5.0 | 5.66 |
| Target | 22.8 | 25.3 | −2.5 | ||