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. Author manuscript; available in PMC: 2018 Jul 11.
Published in final edited form as: Oncogene. 2018 Jan 11;37(11):1457–1471. doi: 10.1038/s41388-017-0049-3

Fig. 5. Intracellular localization of LPA signaling cascade components.

Fig. 5

(A) Representative images of immortalized astrocytes expressing exogenous 3xFLAG-LPAR1, stained for acetylated α-tubulin (AcTub, cilium marker), γ-tubulin (γTub, basal body marker) and FLAG-tag, arrowheads indicate primary cilium; scale bar – 10μm (B) Representative images of immortalized astrocytes stained for acetylated α-tubulin (AcTub), γ-tubulin (γTub), Gαs, Gαq, Gα12 and Gαi1, arrowheads indicate primary cilium; scale bar – 10μm. (C) Immunoprecipitation of 3xFLAG tagged LPAR1 expressed in immortalized astrocytes stably expressing non-targeting shRNA (Con) or shRNA against IFT88 or KIF3B in serum-free conditions. (D) Schematic of potential mechanism of primary cilium restrictive action on the proliferative component of LPA signaling.