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. 2018 Mar 15;8:4641. doi: 10.1038/s41598-018-22848-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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JUN signaling is activated in retinal cells in vivo following intravitreal injection of NMDA. Excitotoxic injury causes JUN activation as represented by pJUN in retinal cells, specifically RGCs, 6 hours after intravitreal injections of either NMDA. Representative images of retinal flat mounts stained with TUJ1 and JUN 6hrs after NMDA injection, showing activation of JUN in TUJ1 + cells (n = 3 for each condition). Scale bar, 50 μm.