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. 2018 Mar 15;8:4577. doi: 10.1038/s41598-018-22874-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of purified VLPs. Figure represents Western blot of (A) HA and (B) M1 proteins in the different VLPs. Total HA and M1 contents in the prepared VLPs were analyzed by SDS-PAGE followed by Western blot using mouse anti-influenza HA and anti-M1 antibodies, respectively, (C) Negative staining electron micrographs of different influenza HA VLPs. Bars represent ∼100 nm.