Fig. 4.

ZEF, ZBF, and EGCG caused the accumulation of the 4T1 cells in the S and G₂/M phases

4T1 cells were treated with 50, 100, 150 and 200 μg/ml ZEF, ZBF, and EGCG for 24 h. The cells were washed, fixed, stained with propidiumiodide, and analyzed for DNA content by flow cytometry. (a) One representative result is shown. (b–d) Percentages of 4T1 cells in different phases of cell cycle after ZEF (b), ZBF (c), and EGCG (d) treatments. ^* P<0.05, ^** P<0.01, ^*** P<0.001 as compared with control (0 μg/ml)