Figure 1.

Autoantibody levels to gangliosides in Zika-infected patients and healthy control individuals. ELISA plates coated with the indicated gangliosides at 20 µg/ml in ethanol, followed by evaporation, were incubated with 1:100 dilution of sera from Zika patients or control healthy individuals and developed with anti-total IgG or anti-Ig-specific subclasses conjugated to HRP. Scatter plots show individual values for each Zika-infected patient (n = 13) and healthy individual (n = 17) for recognition of (A,C) IgG class autoantibodies and (B,D) IgG subclasses (IgG1, IgG2, IgG3, and IgG4 isotypes), against (A,B) total gangliosides (from bovine brain extract) or (C,D) purified ganglioside GD3. Each data point is the mean of triplicated determinations. Means of data points for each individual ± SE are shown. Differences between groups are significant *(p ≤ 0.05), **(p ≤ 0.01), ***(p ≤ 0.01).