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. 2018 Mar 9;9:200. doi: 10.3389/fphar.2018.00200

FIGURE 3.

FIGURE 3

Pharmacological characterization of cAMP-PDE activity in LPS induced THP-1 cells. (A) THP-1 cells were treated with vehicle (0), LPS (1 μg/ml) or LPS + EISO (0.001 or 0.002%) or LPS + IBMX (10 μM) or LPS + rolipram (10 μM) for 4 h. c-AMP PDE activity was determined in the cell lysate as described in Materials and Methods. Data are mean ± SEM (n = 4). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus unstimulated control; †p < 0.05, ††p < 0.01, †††p < 0.001 versus LPS-stimulated cells. (B) The ability of EISO to directly inhibit PDE was assessed in vitro by modification of the cyclic nucleotide phosphodiesterase assay kit as described in Materials and Methods, in vehicle control (0), bovine brain PDE (20 mU) alone, or + IBMX (10 μM), EISO (0.002%), or IBMX + EISO. Data are presented as mean ± SEM (n = 3). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus no PDE control; †p < 0.05, ††p < 0.01, †††p < 0.001 versus PDE alone.