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. 2018 Mar 9;9:419. doi: 10.3389/fimmu.2018.00419

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Copyright © 2018 Tao, Zhang, Ling, McCall and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sirtuin 4 (SIRT4) expression and the mitochondrial bioenergy indices are reprogrammed in human primary blood mononuclear cells during an acute inflammatory response. Healthy peripheral blood mononuclear cells were stimulated with 100 ng/ml lipopolysaccharide (LPS) for indicated times, (A) SIRT3 and SIRT4 gene induction was assessed using real-time-RT-PCR analysis (n = 3). (B) Protein levels of SIRT4 and pyruvate dehydrogenase kinase 1 (PDK1) were detected by western blotting. (C) Cellular energetic changes of OCR to extracellular acidification rate (ECAR) ratio were evaluated using Seahorse XFe-24 analyzer (N = 3). (D) Schematic of mechanism of SIRT4 regulating inflammatory resolution. P values from analysis of variance and post hoc comparisons. Bar graphs depict mean values ± SE of N = 5.