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. 2018 Mar 9;9:467. doi: 10.3389/fimmu.2018.00467

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Copyright © 2018 Zhang, Liu, Xu, Liu, Zhang, Xu, Jia, Li and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Perilipin 1 (PLIN1) overexpression promoted fatty acids and TG synthesis and decreased TG lipolysis in cow adipocytes. Adipocytes were transfected with Ad-PLIN1 and Ad-GFP. Nine replicate samples were used for each condition (N = 9). (A–G) The mRNA expression levels of PLIN1 (A), SREBP-1c (B), ACC1 (C), SCD1 (D), FAS (E), DGAT1 (F), and DGAT2 (G) in adipocytes. (H) Western blot analysis of PLIN1, SREBP-1c, ACC1, SCD1, and FAS. (I–H) The mRNA expression levels of hormone-sensitive lipase (I), CGI-58 for adipose triglyceride lipase (ATGL) (J), and MGLL (K) in adipocytes. (L) The protein expression levels of PLIN1, SREBP-1c, ACC1, FAS, and ATGL. (C) Control group. Abbreviations: Ad-GFP, GFP adenovirus; Ad-PLIN1, PLIN1 overexpression adenovirus. The data presented are the mean ± SEM. (A,B) The same letter indicates no significant difference (p > 0.05), whereas different letters indicate a significant difference (p < 0.05).