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. 2018 Mar 15;8:4630. doi: 10.1038/s41598-018-22879-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TAF1 zinc knuckle binds DNA. (A,B,C) EMSA of TAF1 ZnA (aa 1234–1375) and three radiolabeled DNA fragments: IMD of super core promoter (position −6 to +38), cyclin D1 promoter (position −22 to +29, CD1P), and Random DNA sequence. (D,E,F) Bio-layer interferometry binding curves using biotinylated double-stranded DNA fragments described in above and the following ZnA protein concentrations: 3 μM, 1 μM, 333 nM, 111 nM, 37 nM, 12 nM. Raw data was plotted with GraphPAD Prism. K_d was calculated from plotting steady-state binding levels against protein concentration.