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. 2018 Mar 15;8:4615. doi: 10.1038/s41598-018-21441-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of drought stress (SS: severe stress, MS: moderate stress and WW: well watered) on the production of superoxides (A,B), hydrogen peroxide (C,D) and MDA contents (E,F) in Luhan7 (LH-7) and Yangmai16 (YM-16) wheat cultivars. SS and MS treatments were applied at tillering and jointing growth stage at 35–40% and 55–60% soil field capacity (FC), respectively for ten days followed by re-watering, whereas WW was maintained at 75–80% FC. Time-course of the measurements was one day before stress (0DS), 5^th and 10^th day of stress (5DS, 10DS), 1 and 3 days after re-watering (1DRW, 3DRW). Shaded areas indicate the measurements taken following re-watering. Each vertical bar above the means indicates standard error of six replicates (n = 6) by using two-way ANOVA at P < 0.05.