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. 2018 Mar 15;8:4571. doi: 10.1038/s41598-018-23016-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NMR analysis of secondary structure of hβ4-loop. (a) The chemical shift index (CSI) generated from TALOS program reveals the secondary structure of hβ4-loop. (b) The ¹H-¹⁵N HSQC spectrum (after the ¹⁵N-labeled hβ4-loopsample in H₂O NMR buffer was exchanged into D₂O NMR buffer) indicates the H-bond formed in α-helices and β-sheets in hβ4-loop, assignments of the cross-peaks belonging to the corresponding residues were displayed.