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. 2018 Mar 15;8:4571. doi: 10.1038/s41598-018-23016-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Asn123 of β4 subunit regulates BK channel gating characteristics. (a) Left, G-V curves were plotted for mSlo1, mSlo1/β4, mSlo1/β4(G63A), mSlo1/β4(H105A), mSlo1/β4(N123A), mSlo1/β4(H153A) and mSlo1/β4(D166R) channels. Solid lines were single Boltzmann functions fitted to each G-V curve. Right, the representative current traces from inside-out patches under symmetrical 160 mM K⁺ solution by voltage steps from −140 to + 140 mV with an increment of 20 mV in the presence of intracellular 10 μM Ca²⁺. (b) the V_1/2 values were 30.39 ± 0.57 mV (n = 7), 38.89 ± 0.53 mV (n = 4), −2.53 ± 2.88 mV (n = 9), 42.99 ± 0.58 mV (n = 5), 31.88 ± 1.11 mV (n = 5), 43.91 ± 0.84 mV (n = 5) and 36.96 ± 0.71 mV (n = 5) for mSlo1, mSlo1/β4, mSlo1/β4(N123A), mSlo1/β4(G63A), mSlo1/β4(H105A), mSlo1/β4(H153A) and mSlo1/β4(D166R) channels, respectively. Data were shown as mean ± S.E., and statistical significance was got using two-tailed unpaired Student’s t-test (***P < 0.001); and (c,d) mean activation/deactivation time constants (τ_ac and τ_deac) as functions of membrane voltage were plotted for mSlo1, mSlo1/β4, mSlo1/β4(G63A), mSlo1/β4(H105A), mSlo1/β4(N123A), mSlo1/β4(H153A) and mSlo1/β4(D166R) channels, respectively.