Figure 4.
Five BAA fluorophores were selected for further characterization & multicolor imaging. BAA-37a, BAA-22a, BAA-5a, BAA-2a and BAA-39a were selected for further study. Their (A) chemical structures, absorbance (λmax ABS) and emission (λmax EM) maxima, extinction coefficient (ε) and quantum yield (Φfl) are shown for all five compounds. The normalized (B) absorbance and (C) emission spectra as well as (D) photostability are shown for all five compounds.