Table 2.
Cellular targets and protein labels used for multicolor microscopy.
| Structure | Antigen Label | Secondary Antibody | BAA Fluorophore | Extinction Coefficient (M−1cm−1) | BAA:Protein Conjugation Ratio |
|---|---|---|---|---|---|
| Nuclear Membrane | Anti-lamin A (Abcam, ab8980) | goat anti-mouse IgG3 (msIgG3, 115-005-209) | BAA-37a | 3,500 | 4.2:1 |
| Mitochondial Membrane | Anti-TOMM20 (Abcam, ab78547) | donkey anti-rabbit IgG (rbIgG, 711-005-152) | BAA-22a | 11,200 | 2.4:1 |
| Tubulin | Anti-Tubulin (Millipore, MAB1864) | donkey anti-rat (ratIgG, 712-005-153) | BAA-5a | 24,700 | 4.4:1 |
| Extracellular Membrane | Wheat Germ Agglutinin (Vector Labs, L-102) | N/A | BAA-2a | 126,200 | 0.2:1 |
| Vimentin | Anti-Vimentin (Millipore, AB5733) | donkey anti-chicken (chIgG, 703-005-155) | BAA-39a | 2,600 | 0.9:1 |
*All secondary antibodies were purchased from Jackson Immunoresearch (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA).