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. 2018 Mar 9;9:293. doi: 10.3389/fimmu.2018.00293

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Copyright © 2018 Wang, Zhang, Wu, Wang, Cui, Li, Zhang, Tun, Peng and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NF-κB signaling is negatively regulated by miR-146a in resting and cytokine-stimulated human NK cells. NK-92 cells expressing miR-146a or anti-mIR-146a were cultured in medium without IL-2 for 24 h, followed by treatment with or without IL-12 (10 ng/mL) plus IL-18 (100 ng/mL) for an additional 24 h. Cells were harvested to stain with antibodies, followed by flow cytometric analysis. The NF-κB p65 protein levels and its phosphorylation were measured by flow cytometric analysis (A,B,D,E) (n = 3). The ratio of p-NF-κB to NF-κB was calculated based on mean fluorescence intensity (MFI) of each molecule (C,F) (n = 3). *p < 0.05; **p < 0.01. Error bars represent SD.