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. 2018 Mar 9;9:293. doi: 10.3389/fimmu.2018.00293

Figure 6.

Figure 6

miR-146a expression in resting and IL-12 and/or IL-18-stimulated natural killer (NK) cells. Human NK cells were unstimulated or stimulated with IL-12 (10 ng/mL), IL-18 (100 ng/mL), or the combination of both for 24 h. Supernatants were harvested to be analyzed for IFN-γ production by enzyme-linked immunosorbent assay (ELISA) (A). Cell pellets were collected for RNA preparation and cDNA synthesis, followed by assessment of miR-146a expression by qRT-PCR (B). Human NK cells were unstimulated or co-stimulated with the combination of IL-12 (10 ng/ml) and IL-18 (100 ng/ml) for the indicated times. Supernatants were harvested to be analyzed for IFN-γ production by ELISA (C). Cell pellets were collected and used to prepare RNA and cDNA synthesis, followed by assessment of miR-146a expression by qRT-PCR (D). Data reported are representative of at least four experiments with similar results and are shown as mean ± SD. *p < 0.05; **p < 0.01. Error bars represent SD.