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. 2018 Mar 15;8:4655. doi: 10.1038/s41598-018-23092-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genomic organization of PRL gene. (A) Comparison of the structures of vertebrate PRL genes. The grass carp PRL gene was aligned with that human, chicken, frog (xenopus) and goldfish homologous with respect to the ATG translation start codon at the 3′-end of exon 1. Boxes and lines represent exons and introns, respectively. The scale bar at the bottom represents the distance in kb with respect to the translation start codon ATG. (B) DNA sequences at the intron/exon junctions in different PRL genes. Exon sequences are shown in capital letters and the intron sequences are shown in lower case. The consensus intron splice donor and acceptor sequences are shown in italics.