Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 29;293(2):463–477. doi: 10.1007/s00438-017-1401-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 5 — BSA and subsampling simulations under different sequencing depths and bulk sizes. a |Δ(SNP-index)| at each SNP position across the 14 tetraploid wheat chromosomes, with a detailed plot of chromosome 4B (sequencing depth = 1320×, bulk size = 24). Δ(SNP-index) at each position is calculated by subtracting the SNP-index (mutant allele frequency) in the W-bulk (24 shortest M₄ lines) from that in the M-bulk (24 tallest M₄ lines) [= SNP-index (M-bulk) − SNP-index (W-bulk)]. Blue triangles indicate SNPs with higher frequencies in the mutant bulk [SNP-index (M-bulk) > SNP-index (W-bulk)], while orange circles indicate SNPs with higher frequencies in the W-bulk [SNP-index (W-bulk) > SNP-index (M-bulk)]. Sliding window average (red) is plotted by averaging the |Δ(SNP-index)| values of five consecutive SNPs and shifting the window by one SNP at a time. b Proportion of false positives estimated by counting the number of SNPs with |Δ(SNP-index)| ≥0.8 outside the peak candidate region (chromosome 4B, 12.5–95.3 Mb; Table 1, Online Resource Text S1). (Color figure online)