Fig. 7.

Co-localization of TRPV4, eBK channels, and Cav-1 in the endothelium of gracilis arteries from normoxic controls and CH rats. a Representative confocal images of the Duolink PLA interactions (black puncta). The number of puncta within the borders of the internal elastic lamina was quantified and normalized to the number of observed endothelial cell nuclei (n = 5 rats/group). For negative controls [b], tissue sections were incubated with each primary alone and both PLA probes. Nuclei are labeled with SYTOX. The internal elastic lamina was stained with 633 hydrazide. Bar = 10 μm