Fig. 9.

Representative image of a gracilis artery loaded with Ca²⁺ indicators [a]. Total basal and ACh-induced Ca²⁺ events [b]. Arteries were pretreated with N-nitro-L-arginine (100 μM, L-NNA) and indomethacin (10 μM, INDO). Endothelial cells were selectively loaded with the fluorescent calcium indicators fluo-4 and Oregon Green 488 BAPTA-1. 600 images/artery were collected at 50–60 Hz using a spinning disk confocal microscope (× 40 oil). ACh-induced Ca²⁺ events were assessed in arteries pretreated with vehicle or the TRPV4 antagonist GSK2193874 (300 nM). *Different from baseline or baseline GSK219 within group (P < 0.05). ^#Different from ACh within group (P < 0.05). ^&Different from normoxic baseline and normoxic baseline GSK219 (P < 0.05). ^%Different from normoxic ACh (P < 0.05). N = 5–6/group