Fig. 4.

CycinD1 was significantly suppressed upon MEN1/menin knockdown. a The expression of cell cycle progression associated genes was assessed in the MAC-T cells after 24, 48 and 72 h of transfection with MEN1-specific siRNAs, as well as their negative controls. The dotted line represents the expression levels of each genes in their negative control cells at the indicated time points. Cyclin D1 expression was significantly inhibited all time points. **P < 0.01, *P < 0.05. b The suppression of Cyclin D1 mRNA expression was confirmed by three independent MEN1 knockdown experiments at 24 h posttransfection. The data are shown as relative expression levels normalized to the internal control β-actin. *P < 0.05. c Cyclin D1 protein expression was suppressed upon MEN1/menin knockdown (n = 3). *P < 0.05. d Representative western blot images of the expression of menin, Cyclin D1 and the loading control β-actin are shown