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. 2017 Aug 23;43(5):987–1000. doi: 10.1038/npp.2017.144

Figure 2.

Figure 2

Fos expression and functional inhibition of lateral septum (LS) neurons during context- and cue-induced reinstatement of cocaine seeking. (a) Location of LS in a rat atlas coronal section (0.72 mm anterior to bregma; Paxinos and Watson, 2007) and (b) representative photomicrographs of Fos expression in LS during a cue- or (c) context-induced reinstatement test. Scale bars, 100 μm. Whole images adjusted for contrast and brightness in representative tissue for cue-induced reinstatement. (d) Context reinstatement (dark blue bars, n=8) induced a greater number of Fos+ cells compared to cue-induced reinstatement (light blue bars, n=6) in rostral LS (*P<0.05) or (e) compared to cue-induced reinstatement or yoked saline rats (gray bars, n=5) in caudal LS (*P<0.05). White bars represent extinction group, n=5. SEM indicated by error bars. (f) Representative photomicrograph of cannula injection sites in LS (Nissl stain, frontal section, dorsal up, midline at center). Brain atlas inset shows bilateral injection sites across all rats (black circles; n=12). Scale bar, 200 μm. (g) Microinjections of the inhibitory cocktail baclofen plus muscimol (B-M, solid blue bar) into LS attenuated context-induced reinstatement (*P<0.05) and cue-induced reinstatement relative to vehicle/aCSF (open blue bar) microinjections (**P<0.01). n.s.=not significant.