Figure 2.
Effects of ICV NPS (1 nmol/5 μl) on relative amount of phosphorylated CaMKIIα (pCaMKIIα) compared with (a) total CaMKIIα and (b) β-tubulin in the cytosolic fraction of amygdala tissue micropunches of male rats. NPS evoked synthesis of (c) CaMKIIα in relation to β-tubulin. Relative amount of phosphorylated ERK1/2 (pERK1/2) compared with (d) total ERK1/2 and (e) β-tubulin without affecting (f) total ERK1/2 levels relative to β-tubulin following NPS infusion. Data represent mean+SEM, *p<0.05 versus Veh; group sizes: n=9–12. (g) Representative western blots of (p)CaMKIIα, (p)ERK1/2, and β-tubulin.
