Figure 3.

Effects of preinfusion of an inhibitor-cocktail (Inh-cock) consisting of both U73122 (PLC inhibitor, 0.5 nmol) and DDA (AC inhibitor, 12.5 nmol) on the anxiolytic effect of NPS bilaterally infused into the MeA (0.2 nmol/0.5 μl) on the EPM (a–c) and OF (d–f). (a) Percentage of time spent on the EPM, (b) percentage of entries performed into the open arms, (c) closed arm entries, and (d) time spent in the OF, (e) entries performed into the center zone, and (f) distance traveled. As Veh₁, 0.5 μl of Ringer’s solution supplemented with DMSO, and as Veh₂, 0.5 μl of Ringer’s solution were used. Data represent mean+SEM, **p<0.01, ***p<0.001 versus all other groups (a, e, f) or as indicated; group sizes: n=13–15, except Inh-cock/Veh₂: n=6–8.