Fig. 3. Effect of antidepressants on adipogenesis. Human mesenchymal stem cells were induced to differentiate into adipocytes for 2 weeks at 37℃ in the presence of various concentrations of (A, B) amitriptyline, (C, D) venlafaxine, or (E, F) fluoxetine. Differentiating cells were also incubated with vehicle (VEH), while undifferentiated cells were incubated with MSC growth media (GM) for 2 weeks as controls. Cells were then fixed, stained with oil red O (ORO) and counterstained with hematoxylin as described in the methods. The extent of staining was then quantified by solubilizing the stain as described in the methods. Intensity of staining was determined by absorbance relative to VEH. Data are representative of 3 to 6 independent experiments per drug. Images were taken under ×10 magnification. ^*P<0.05 compared to VEH-treated cells.