Fig. 4. Assessment of cell viability. Human mesenchymal stem cells (MSC) induced to differentiate into osteoblasts (alizarin red [AR]) or adipocytes (oil red O [ORO]) in the presence of various concentrations of amitriptyline (Ami), venlafaxine (Ven), or fluoxetine (Fl). The Figure shows the effect of increasing concentrations of the drugs on cell viability. No significant effect was found at any treated conditions. Cells cultured in MSC growth media (GM) were used as control. ^*P<0.05 compared to differentiation media. VEH, vehicle.