FIG 3.

The mouse and human SULT2B1b genes are transcriptional targets of HNF4α. (A) Sequences of the predicted HNF4α binding sites in the mouse and human SULT2B1b gene promoters and their mutant variants (top). The bindings of HNF4α to the putative HNF4α binding sites were shown by electrophoretic mobility shift assay (EMSA) (bottom). (B and C) ChIP assay showing the recruitment of HNF4α onto the mouse (C) and human (D) SULT2B1b gene promoters. (D and E) Activation of the mouse (D) and human (E) SULT2B1b promoter reporter genes by HNF4α. Cells were cotransfected with the indicated WT or mutant reporter genes, and receptors were monitored by luciferase assay. Results are expressed as means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01.