Figure 2.

Secretion of IGFBP1 and PRL from normal resistance index (RI) and high RI DSC. Isolated DSC, from normal RI and high RI pregnancies, were stimulated with cAMP and MPA to stimulate decidualisation, after 72 h conditioned media was collected and levels of IGFBP1 and PRL measured by ELISA. Secretion of IGFBP1 was significantly increased in re-decidualised (rDSC) compared to DSC from normal RI (A) (n = 33) and high RI (B) (n = 15) pregnancies. Levels of PRL were significantly increased in rDSC compared to DSC CM from normal RI (C) (n = 25) and high RI (D) (n = 19) pregnancies. Secretion of (E) IGFBP1 or (F) PRL from rDSC from normal RI and high RI did not differ. Data were log transformed to ensure equal variance. Paired t-test (A, B, C and D), unpaired t-test (E and F), data shown as mean ± s.e.m., ****P < 0.001 and **P < 0.01, ns denotes not significant.