Figure 4.

Effect of rDSC CM from normal and high RI pregnancies on chemotaxis of SGHPL-4 cells. SGHPL-4 cells were cultured in chemotaxis chambers to analyse chemotactic capacity of normal and high RI rDSC CM. Non-directional movement of cells gives expected chemotaxis of 50%. (A) Normal RI rDSC CM from pregnancies <10 weeks gestation is significantly more chemotactic to SGHPL-4 cells than high RI rDSC CM from pregnancies at <10 weeks gestation. (B) Normal and high RI rDSC CM from pregnancies >10 weeks gestation have no chemotactic effect on SGHPL-4 cells (n = 6). Paired t-test, data shown as mean ± s.e.m., *P < 0.05.