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. 2018 Mar 13;11(3):575–584. doi: 10.1016/j.tranon.2018.02.003

Figure 1.

Figure 1

Expression and function of DDR1 in GC. (A-B) Expression of DDR1 in human GC cell lines. Expression levels of DDR1 mRNA in GC cells were quantified by qRT-PCR (n=3) (A) and Western blot (B). (C-E) Generation of DDR1 knockdown in MKN74 cells. MKN74 cells were transfected with shDDR1 and scrambled shRNA lentiviral particles. At 48 hours postinfection, cells were selected with puromycin. DDR1 knockdown was confirmed by qRT-PCR (n=3) (C) and Western blot analysis (D). (E) Immunoprecipitation (IP) of DDR1 from cell lysates of MKN74 after incubation with or without collagen I (15 μg/ml) for 3 hours. The blots were probed with anti-phosphotyrosine antibody and subsequently reprobed with anti-DDR1. (F-H) Effects of DDR1 knockdown on proliferation, migration, and invasion. (F) No differences were observed in cell proliferation following DDR1 knockdown. (G-H) Cells transduced with DDR1 shRNA exhibited significantly inhibited migration and invasion compared to control cells. All assays were measured using IncuCyte technology, which monitors cell activity in real time (one image every 2 hours) for a period of 3 to 5 days.