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. 2018 Mar 13;11(3):575–584. doi: 10.1016/j.tranon.2018.02.003

Figure 2.

Figure 2

Effect of DDR1 silencing on MKN74 orthotopically implanted tumor growth. Histological and immunofluorescent staining of orthotopic tumors at 28 days after cell implantation. (A) Hematoxylin and eosin staining. DDR1-silenced implanted tumors grew expansively, and necrosis was observed at the tumor center (right). (B) DDR1 expression was attenuated in tumors transfected with DDR1 shRNA (right). (C) In control tumors, there were rich collagen bands, and phosphorylated DDR1 was detected in cancer cells (left). Type I collagen: green, p-DDR1: red, DAPI: blue. (D, E) Mean densities of lymphatic and blood vessels were significantly reduced in DDR1-silenced tumors. The insets show high-magnification images of Ki-67 signal detected in the vessels of control tumors. There was no Ki-67 signal detected in the vessels of DDR1-silenced tumors. Ki-67: red, Lyve1 and CD31: green. (F) Analysis of cell proliferation (Ki-67). There was no significant difference in the Ki-67 proliferation indexes. Data are expressed as means ± SEM. **P < .01.