Figure 6.

Time-dependent effect of RTD-1 on airway neutrophilia and cytokines. To assess timing of drug administration, 25 mg/kg RTD-1 was administered at 0 or 12 hours after 1-μg LPS instillation. As a comparator agent, 2 mg/kg dexamethasone (Dex) was given at 0 hour. LPS mice administered saline served as control. (A) BALF WBCs were quantified by manual counting. BALF concentrations of TNF (B), IL-1β (C), IL-6 (D), inducible protein 10 kD (IP-10) (E), and IFN-γ (F) were quantified by multiplex ELISA; n = 9 mice per group (except n = 8 for RTD-1 at 0 h); mean (±SD). Treatment compared with control by unpaired two-sided t test *P < 0.05, **P < 0.01, ***P < 0.001.