Figure 7.

RTD-1 effects on severity of acute lung injury. To assess treatment effects on lung injury, mice received pretreatment with 0 or 25 mg/kg RTD-1 0.5 hour before 1-μg LPS insufflation. Lungs were formalin inflated and hematoxylin and eosin stained. Representative photomicrographs of each mouse and their respective treatment groups (A–C) LPS alone, (D–F) LPS + 25 mg/kg RTD-1, or (G–I) PBS sham are shown. (J) Pathological scoring of slides for lung injury and inflammation. Parameters included the presence of congestion, interstitial and alveolar leukocytes, and alveolar hyaline membranes; n = 3 mice per group; mean (±SD). Treatment compared with control by unpaired two-sample t test. Scale bars: 100 μm. Magnification ×10. **P < 0.01.