Figure 4.

BN-PAGE-based in-gel activity of individual mitochondrial respiratory complexes from gastrocnemius muscle of N, HFD, and HFD+T2 rats. (A) In-gel activity of complex I. (B) In-gel activity of complex II. (C) In-gel activity of complex IV. (D) In-gel activity of complex V. Protein extracts were prepared for each animal, and each individual was assessed separately. Data were normalized to the value obtained for N animals, set as 1, and presented separately for each treatment (means ± SD; n = 6). Bars labeled with dissimilar letters are significantly different (P < 0.05).