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. 2018 Mar 9;9:194. doi: 10.3389/fphys.2018.00194

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Copyright © 2018 Silvestri, Cioffi, De Matteis, Senese, de Lange, Coppola, Salzano, Scaloni, Ceccarelli, Goglia, Lanni, Moreno and Lombardi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of HFD and T2-treatment on mitochondrial H₂O₂ release and protein carbonylation. (A) Mitochondrial H₂O₂ release (Delta F/min) in gastrocnemius muscle mitochondria of N, HFD and HFD+T2 rats. Bars labeled with dissimilar letters are significantly different (P < 0.05). (B) Representative immunoblot showing protein carbonylation in gastrocnemius muscle of N, HFD, and HFD+T2 rats. (C) Representative density traces for carbonylated protein bands are also reported.