Genetic differentiation at 23 microsatellite loci across the genome on the basis of FST, RST, D′, and DK. A compares A. gambiae and A. arabiensis, whereas B compares the M and S molecular forms of A. gambiae at 25 loci, the first two of which cannot be amplified for A. arabiensis. Note that the two A. gambiae forms are practically indistinguishable except at loci H678 and E614, where they are clearly distinct (see Fig. 1B). The numbers of sampled alleles are shown at each locus. Bars represent genetic distance values in red (“clearly different”), yellow (“marginal”), or green (“indistinguishable”), according to the following criteria. For DK, Pt and Pf are at >10%, between 2.5 and 10% or at <2.5% probability, respectively; for D′ and FST, Pd and Ps are at <2.5%, between 2.5 and 10% or at >10% probability, respectively; for RST, the value of Nm is <0.5, between 0.5 and 3 or >3, respectively.