REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-CYFIP2	Abcam	Cat# ab79716, RRID: AB_10673468
anti-CYFIP1	Abcam	Cat# ab108220, RRID: AB_10859239
anti-NCKAP1	Abcam	Cat# ab140856, RRID: AB_2721067
anti-xFXR	Huot et al., 2005	N/A
anti-ELAVLs	Santa Cruz Biotechnology	Cat# sc-5261, RRID: AB_627770
Anti-RPL10a	Proteintech Group	Cat# 16681-1-AP, RRID: AB_2181281
Anti-RPS3A	Proteintech Group	Cat# 14123-1-AP, RRID: AB_2253921
anti-puromycin	Millipore	Cat# MABE343, RRID: AB_2566826
anti-tubulin	Sigma-Aldrich	Cat# T6074, RRID: AB_477582
Anti-GFP	Abcam	Cat# ab6556, RRID: AB_305564
HRP-conjugated secondary antibody	Abcam	Cat# ab6789, RRID: AB_955439
HRP-conjugated secondary antibody	Abcam	Cat# ab97080, RRID: AB_10679808
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide	Sigma-Aldrich	Cat# C4859
Rapamycin	Calbiochem	Cat# 553210
Cytochalasin D	Sigma-Aldrich	Cat# C8273
Cy3-UTP	PerkinElmer	Cat# NEL582001EA
1,1’-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate (Dil)	Thermo Fisher Scientific	Cat# D282
3,3′-dioctadecyloxacarbocyanine perchlorate (DiO)	Thermo Fisher Scientific	Cat# D275
Puromycin	Sigma-Aldrich	Cat# P8833
Dynabeads Protein G	Life Technologies	Cat# 10004D
Critical Commercial Assays
mMESSAGE mMACHINE SP6 Transcription Kit	Thermo Fisher Scientific	Cat# AM1340
Gibson Assembly cloning kit	New England BioLabs	Cat# E5510S
QuikChange II Site-directed Mutagenesis Kit	Agilent Technologies	Cat# 200555
RNeasy Mini Kit	QIAGEN	Cat# 74104
MEGAscript T7 transcription Kit	Thermo Fisher Scientific	Cat# AM1334
RNA clean & concentrator-5	Zymo Research	Cat# R1016
Phire Animal Tissue Direct PCR Kit	Thermo Fisher Scientific	Cat# F140WH
DUOlink in situ PLA kit	Sigma-Aldrich	Cat# DUO92014
Experimental Models: Organisms/Strains
Xenopus laevis	Nasco	https://www.enasco.com/p/LM00715MX/; https://www.enasco.com/p/LM00535MX/
Zebrafish: Tg(atoh7:gapGFP)	Zolessi et al., 2006	ZFIN: ZDB-ALT-070129-1
Zebrafish: zTOR	Ding et al., 2011	ZFIN: ZDB-ALT-120412-1
Oligonucleotides
Morpholino: Xenopus CYFIP2 TTACCAAGTCCGGTAGCGACAGTCT	Gene Tools	N/A
Morpholino: Control CCTCTTACCTCAGTTACAATTTATA	Gene Tools	N/A
Morpholino: zebrafish CYFIP2 AGTGCATTAGGACGTGTACCTGGTA	Gene Tools; Pittman et al., 2010	N/A
CYFIP1gRNA: GGAGGGCAGAGGCTCGATGC	Sigma-Aldrich	N/A
CYFIP2gRNA: GACAACCCACGTGACCCTGG	Sigma-Aldrich	N/A
Primer: sequencing zebrafish CYFIP1 5′ GCCATGTCTCACATGTGTTTTT 3′ 5′ GCAGATACAGAAGAAGGGTTGC 3′	Sigma-Aldrich	N/A
Primer: sequencing zebrafish CYFIP2 5′ AGGTCATGACATTTCCCTTGTC 3′ 5′ TCAGTGCATTAGGACGTGTACC 3′	Sigma-Aldrich	N/A
Recombinant DNA
pCS2+mGFP	Das et al., 2003	N/A
pCS2+mRFP	Poggi et al., 2005	N/A
pCS2+CYFIP2-GFP	This paper	N/A
pCS2+CYFIP2-CTD-GFP	This paper	N/A
pCS2+CYFIP2mutE-GFP	This paper	N/A
pCS2-Cas9	Addgene	Cat# 47322
Xenopus CYFIP2	Source Bioscience	IRBHp990E0729D
CYFIP1	Dharmcon/GE Healthcare	MHS6278-202827458
CYFIP2	Source Bioscience	IRAUp969A0861D
Software and Algorithms
Volocity	v.6.3.1	Volocity 3D Image Analysis Software, RRID: SCR_002668
Fiji	v.2.0.0-rc-43/1.51n	Fiji, RRID: SCR_002285
GraphPad PRISM	v.6.01	GraphPad Prism, RRID: SCR_002798
ND-SAFIR	Boulanger et al., 2010	http://serpico.rennes.inria.fr/doku.php?id=software:nd-safir:index