Skip to main content
. Author manuscript; available in PMC: 2018 Jun 14.
Published in final edited form as: Cell Host Microbe. 2017 Jun 14;21(6):731–741.e10. doi: 10.1016/j.chom.2017.05.006

Figure 4. Only PfAP2-I-D3 is required for DNA-binding during the IDC.

Figure 4

A- The mutations introduced in the AP2 domains resulting in PfAP2-I-GFP-D1mut and PfAP2-I-GFP-D2mut parasites (see also figures S5A-D). B- ChIP-qPCR with PfAP2-I-D2mut parasites shows that PfAP2-I still binds to its target genes when D2 is mutated (blue bars). PfAP2-I-GFP parasites were used as control (black bars). Data are represented as mean ± SD and n=3. C- PBMs show that the DNA-binding of PfAP2-I AP2 domains D1, D2 and D3 are altered when the domains are mutated. Shown are both wt and mutant PBM results. Enrichment values below 0.45 represent low affinity DNA-binding (see Table S6 for position weight matrices for all AP2 domains).