Skip to main content
. Author manuscript; available in PMC: 2018 Jun 14.
Published in final edited form as: Cell Host Microbe. 2017 Jun 14;21(6):731–741.e10. doi: 10.1016/j.chom.2017.05.006
Dd2 wt strain grown in standard culture media
PfAP2-I-GFP Dd2 strain with endogenous PfAP2-I C-terminally tagged with GFP grown in standard media supplemented with BSD
PfSIP2N-HA 3D7 strain expressing episomal copy of PfSIP2 N-terminus fused to 3xHA grown in standard media supplemented with BSD (parasite line previously generated)
PfAP2-I-GFP::msp5MUT PfAP2-I-GFP Dd2 strain with mutated PfAP2-I DNA motif in the promoter of endogenous msp5 grown in standard media
PfAP2-I-GFP-D1mut PfAP2-I-GFP Dd2 strain with mutated AP2 domain 1 (D1) grown in standard media
PfAP2-I-GFP-D2mut PfAP2-I-GFP Dd2 strain with mutated AP2 domain 2 (D2) grown in standard media
PfAP2-INLS-GFP Dd2 strain expressing episomal copy of NLSPfAP2-I-GFP grown in standard media supplemented with WR
NLSPfSIP2-GFP Dd2 strain expressing episomal copy of NLSPfSIP2-GFP grown in standard media supplemented with WR
NLSCalmodulin-YFP 3D7 strain expressing episomal copy of NLSCalmodulin-YFP grown in standard media supplemented with WR (parasite line previously generated)
HDGFP 3D7 strain expressing episomal copy of NLSCalmodulin-YFP grown in standard media supplemented with WR (parasite line previously generated)
PfAP2-I-GFP::PfBDP1-HA-DD PfAP2-I-GFP Dd2 strain with endogenous PfBDP1 C-terminally tagged with 3xHA and FKBP (DD) grown in standard media supplemented with WR and BSD